Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of the most significant developments in optics. Due to the wave nature of light ...

To unravel the complexities of biological phenomena, scientists have long relied on microscopy to visualize the intricate details of their specimens, including tissue architecture, cell morphology, ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

When single-molecule super-resolution microscopes were first commercialized some 15 years ago, they made headlines for their ability to resolve individual molecules and structures at the nanometer ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

This is especially true when studying intricate details beyond the optical resolution limit using structured illumination microscopy (SIM). SIM uses multiple images acquired under structured ...