STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to achieve an improvement in resolution, that is …

Stimulated emission depletion (STED) microscopy uses spatially modulated and saturable transitions between two molecule states to create superresolution images.

Researchers have used STED to image the fine structures within neurons, including the distribution of synaptic proteins and the organization of synaptic vesicles, which are tiny sacs involved in …

Stimulated emission depletion (STED) microscopy is a super-resolution microscopy technique that enhances resolution by selectively de-exciting fluorophores at the periphery of the focal point.

STED microscopy uses two laser pulses to localize fluorescence at each focal spot. The first pulse is used to excite a fluorophore to its fluorescent state, and the second pulse is a modified beam used to …

Aug 8, 2024 · In this Primer, we focus on the most recent advancements in stimulated emission depletion (STED) microscopy, encompassing optics, computational microscopy and probes design, …

Stimulated emission depletion (STED) microscopy is a far-field fluorescence imaging technique that fundamentally breaks the diffraction barrier. In a STED microscope, the spatial resolution can be …

The STED donut determines where the fluorescence originates in space; therefore, imaging with a single STED line ensures that the different channels (colors) are intrinsically aligned.

Jun 8, 2025 · In this series of articles on STED microscopy, I will cover more about how it works, optimising staining, and share some tips and tricks on how to get the most out of it.

Stimulated Emission Depletion (STED) microscopy is a fluorescence microscopy super-resolution technique that is able to circumvent the optical diffraction limit. STED microscopy was first described …